CST, 59352T, S-Tag (D2K2V) Mouse Chimeric Monoclonal Antibody

Monoclonal Antibody for studying . Validated for Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, S-Tag (D2K2V) Mouse Chimeric Monoclonal Antibody (CST #59352) is ready to ship. Product Usage Information Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity S-Tag (D2K2V) Mouse Chimeric Monoclonal Antibody recognizes transfected levels of recombinant protein containing an S-Tag. This antibody detects exogenously expressed S-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. Species Reactivity: All Species Expected Source / Purification This recombinant chimeric antibody is engineered from S-Tag (D2K2V) XP Rabbit mAb #12774 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies. The parent antibody, S-Tag (D2K2V) XP Rabbit mAb #12774, is produced by immunizing animals with a synthetic S-Tag peptide. Background Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. S-Tag is an epitope tag composed of a 15 residue peptide that, together with S-protein, comprises pancreatic ribonuclease S (1). Specification REACTIVITY: All SENSITIVITY: Transfected Only Source/Isotype: Mouse chimeric IgG2a