CST, 5935T, BRSK1 (D10F2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BRSK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BRSK1 (D10F2) Rabbit Monoclonal Antibody (CST #5935) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BRSK1 (D10F2) Rabbit Monoclonal Antibody detects endogenous levels of total BRSK1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly485 of human BRSK1 protein. Background ain- pecific inase (BRSK1; SAD-B) and ain- pecific inase 2 (BRSK2; SAD-A) are serine/threonine kinases closely related to AMPK. LKB1 phosphorylates Thr189 in the T-loop of BRSK1 and Thr174 in the T-loop of BRSK2, resulting in activation of the kinases (1). BRSK1 localizes to synaptic vesicles in the hippocampus and cerebellum, together with the active zone proteins Bassoon and CAST, and BRSK1 phoshorylates the active zone protein RIM1 (2). An alternatively spliced from of BRSK1 displays unique activity during the cell cycle, phosphorylating Ser131 of γ-tubulin and controling centrosome duplication (3). Neuronal polarization, including axon formation, is fundamental for normal brain development. BRSK1 -/- and BRSK2 -/- mice have defects in neuronal polarity and impaired corticogenesis (4). Knockdown of BRSK1 and BRSK2 diminishes axonal growth (5). Alternate Names BR serine/threonine kinase 1; BR serine/threonine-protein kinase 1; Brain-selective kinase 1; Brain-specific serine/threonine-protein kinase 1; BRSK1; FLJ43009; hSAD1; KIAA1811; protein kinase SAD1A; SAD1; SAD1 homolog; SAD1 kinase; SADB; Serine/threonine-protein kinase BRSK1; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG