CST, 5940T, Mili (D14F5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Mili. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Mili (D14F5) Rabbit Monoclonal Antibody (CST #5940) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:100 Immunofluorescence (Frozen): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen) Specificity / Sensitivity Mili (D14F5) Rabbit Monoclonal Antibody recognizes endogenous levels of total Mili protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Leu118 of mouse Mili protein. Background The piwi gene was identified as being required for the self-renewal of germ-line stem cells (1). Piwi homologs are well conserved among various species including , and human (1). Miwi and Mili proteins are both mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mouse (3-5). Alternate Names CT80; Mili; Miwi like; Miwi likw; piwi like homolog 2; piwi-like homolog 2; piwi-like homolog 2 (Drosophila); Piwi-like protein 2; piwi-like RNA-mediated gene silencing 2; Piwil1l; Piwil2; PIWL2 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG