CST, 5942S, TDRD3 (D3O2G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TDRD3. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TDRD3 (D3O2G) Rabbit Monoclonal Antibody (CST #5942) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TDRD3 (D3O2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total TDRD3 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TDRD3 protein. Background Tudor domain-containing protein 3 (TDRD3) contains a tudor domain through which it binds to asymmetric di-methyl histone H3 (Arg17) and asymmetric di-methyl histone H4 (Arg3). Both of these histone marks are associated with transcription activation (1,2). TDRD3 is targeted to estrogen responsive genes where it performs as a coactivator (1). TDRD3 acts as a scaffolding protein to recruit Topoisomerase III B (TOP3B) to target genes to reduce transcription-generated R loops by relaxing negatively supercoiled DNA thereby reducing genomic instability (3). In addition to the nucleus, TDRD3 also resides in the cytoplasm where it associates with actively translating polyribosomes and accumulates into stress granules upon exposure to stress, implicating its role in post-transcriptional regulation of RNA (2,4). Alternate Names FLJ21007; TDRD3; tudor domain containing 3; Tudor domain-containing protein 3 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 83 Source/Isotype: Rabbit IgG