CST, 59500T, NPM1 (C Mutant Specific) (F7R1P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NPM1-C mutation. Validated for WB,IP,IF,F,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, NPM1 (C Mutant Specific) (F7R1P) Rabbit Monoclonal Antibody (CST #59500) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP Specificity / Sensitivity NPM1 (C Mutant Specific) (F7R1P) Rabbit Monoclonal Antibody recognizes endogenous levels of total NPM1 C mutant protein. This antibody may also detect a band around 220 kDa that likely corresponds to pentameric NPM1 C mutant protein. This antibody does not cross-react with wild-type NPM1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the mutated carboxy terminus of human NPM1 C mutant protein. Background Nucleophosmin (NPM1), also known as NPM, B23, numatrin, or NO38, is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication, and molecular chaperoning activities (1,2). The gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM1 portion contributes to transformation by providing a dimerization domain, which activates the fused kinase (3,4). is also the most frequently mutated gene in acute myeloid leukemia (AML) and accounts for nearly 30% of all cases (5). These AML subtypes, classified as -mutated AML, are characterized by mutations in NPM1's C-terminus that disrupt its nucleolar localization sequence and cause mislocalization from the nucleolus to the cytoplasm (6). This cytoplasmic form of NPM1, commonly referred to as NPM1c, is exclusive to myeloid malignancies and is not found in other forms of cancer (7). These mutations are always heterozygous, and NPM1c functions in a dominant negative fashion by dimerizing with wild-type NPM1 and recruiting it to the cytoplasm (6,8). Interestingly, mutations alone are not sufficient to drive leukemogenesis, and further research is required to fully elucidate the impact of these mutations on disease progression (9). Alternate Names B23; MGC104254; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin 1; nucleophosmin/nucleoplasmin family, member 1; Numatrin; testicular tissue protein Li 128 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG