CST, 59581S, HDAC1 (10E2) Mouse Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying HDAC1. Validated for Western Blotting. Highly specific and rigorously validated in-house, HDAC1 (10E2) Mouse Monoclonal Antibody (HRP Conjugate) (CST #59581) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibodies. Protocol Available protocols: Western Blotting Specificity / Sensitivity HDAC1 (10E2) Mouse Monoclonal Antibody (HRP Conjugate) detects endogenous levels of total HDAC1 protein. This antibody conjugate does not cross-react with other HDAC proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human HDAC1 protein. Background Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). Alternate Names DKFZp686H12203; GON-10; HD1; HDAC 1; HDAC1; Histone deacetylase 1; KDAC1; reduced potassium dependency, yeast homolog-like 1; RPD3; RPD3L1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Mouse IgG1