CST, 59679SF, SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying FPN. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #59679) is ready to ship. Product Usage Information This product is the carrier free version of product #48322. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 48322 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity SLC40A1/Ferroportin-1 (F9U5S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total SLC40A1/Ferroportin-1 protein. Non-specific staining was observed in pancreatic acinar cells by immunohistochemistry. This antibody is not recommended for immunohistochemical analysis of mouse tissues. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu265 of human SLC40A1/Ferroportin-1 protein. Background Ferroportin-1 (FPN1), also known as solute carrier family 40 member 1 (SLC40A1) or iron-regulated transporter 1 (IREG1), is a multi-pass membrane protein that transports iron from the inside of the cell into the blood. As the only known iron exporter, FPN1 is critical for maintaining systemic iron homeostasis (1). FPN1 activity is regulated by hepcidin, a peptide hormone secreted by the liver in response to iron loading and inflammation. Circulating hepcidin binds to FPN1 and induces its internalization and degradation, thereby reducing iron efflux to the plasma (2,3). Expression of FPN1 may also be regulated post-transcriptionally by several microRNAs that directly target its 3' untranslated region (4-6). Mutations in the gene encoding FPN1 are associated with type IV hemochromatosis (7) as well as several neural tube and patterning defects, including spina bifida, exencephaly, and forebrain truncations (8). Loss of FPN1 in the brains of Alzheimer's disease (AD) mouse models and patients has been shown to promote ferroptosis, leading to neuronal death and memory impairment. Therefore, targeting of FPN1 may be a promising therapeutic approach for AD (9). Alternate Names ferroportin 1; Ferroportin-1; FPN1; HFE4; IREG1; iron regulated gene 1; Iron-regulated transporter 1; MST079; MSTP079; MTP1; putative ferroportin 1 variant IIIB; S40A1; SLC11A3; SLC40A1; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3; solute carrier family 40 (iron-regulated transporter), member 1; Solute carrier family 40 member 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 62 Source/Isotype: Rabbit IgG