CST, 59698SF, Rhodopsin (D4B9B) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying rhodopsin. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Rhodopsin (D4B9B) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #59698) is ready to ship. Product Usage Information This product is the carrier free version of product #27182. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 27182 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Rhodopsin (D4B9B) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total rhodopsin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu194 of human rhodopsin protein. Background Rhodopsin is the photoreceptor in the retinal rods. It is activated by photons, transduces visual information through its cognate G protein, transducin, and is inactivated by arrestin binding (1). Using atomic-force microscopy, rhodopsin was found to be arranged into paracrystalline arrays of dimers in mouse disc membranes (2). Rhodopsin is considered to be the prototype of G protein-coupled receptors (GPCRs), and is the first GPCR for which a crystal structure was solved (3). Research studies have linked mutations in the gene encoding rhodopsin to retinitis pigmentosa (4,5), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness (6). Alternate Names CSNBAD1; MGC138309; MGC138311; OPN2; OPSD; opsin 2, rod pigment; Opsin-2; retinitis pigmentosa 4, autosomal dominant; RHO; Rhodopsin; rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant); RP4 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG