CST, 59710T, TDP1 (D8D1B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TDP1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, TDP1 (D8D1B) Rabbit Monoclonal Antibody (CST #59710) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TDP1 (D8D1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total TDP1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro587 of human TDP1 protein. Background Topoisomerases are ubiquitous, conserved enzymes that remove DNA supercoils resulting from processes such as chromosome segregation, DNA replication, transcription, and repair (1). Topoisomerase inhibitors such as camptothecin and etoposide trap the enzyme as a DNA-bound intermediate, and these drugs are used to treat multiple human cancers (1,2). Tyrosyl-DNA-phosphodiesterases TDP1 and TDP2 function in the base excision repair (BER) and nonhomologous end joining (NHEJ) DNA repair pathways, respectively, and function in part in the repair of stalled topoisomerase-DNA complexes (3). Research has shown that inhibitors of tyrosyl-DNA-phosphodiesterases may act synergistically with topoisomerase inhibitors, allowing the potential for a more robust treatment of cancer (4,5). In small cell lung cancer, research suggests that TDP1 and topoisomerase 1 levels can predict sensitivity to topoisomerase 1 inhibitors (6). Alternate Names FLJ11090; MGC104252; TDP1; TYDP1; Tyr-DNA phosphodiesterase 1; Tyrosyl-DNA phosphodiesterase 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG