CST, 59730S, MASTL (F7I2C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MASTL. Validated for WB,WB,IP,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, MASTL (F7I2C) Rabbit Monoclonal Antibody (CST #59730) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity MASTL (F7I2C) Rabbit Monoclonal Antibody recognizes endogenous levels of total MASTL protein. This antibody detects an approximately 190 kDa protein of unknown identity in some cell lines. Non-specific cytoplasmic staining was observed in brain by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg592 of human MASTL protein. Background Mitotic control is important for normal growth, development, and maintenance of all eukaryotic cells. Research studies have demonstrated that inappropriate control of mitosis can lead to genomic instability and cancer (reviewed in 1,2). A regulator of mitosis, Greatwall kinase (Gwl), was first identified in (3). Subsequent studies showed that, based on sequence homology and function, microtubule-associated serine/threonine kinase-like (MASTL) is the human ortholog of Gwl (4). Regulation of MASTL/Gwl activation has been shown to be critical for the correct timing of mitosis. Research studies have shown that Gwl is activated by hyperphosphorylation (5). The phosphorylation of human Gwl at Thr194 and Thr207 by active cyclin B1-cdc2 leads to possible autophosphorylation at Ser875 (Ser883 in ), which stabilizes the kinase. Activated Gwl phosphorylates α-Endosulfine (ENSA) and cAMP-regulated phosphoprotein 19 (ARPP19) at Ser67 and Ser62, respectively. Phosphorylated ENSA and ARPP19 inhibit the activity of the B55 subunit-associated form of protein phosphatase 2A (PP2A-B55), allowing for complete phosphorylation of mitotic substrates by cyclin B1-cdc2 and mitotic entry. When Gwl is inactivated, PP2A-B55 reactivates, which leads to dephosphorylation of cyclin B1-cdc2 and mitotic exit (5,6, reviewed in 7). Alternate Names FLJ14813; greatwall; greatwall kinase homolog; greatwall protein kinase; GW; GWL; hGWL; MAST-L; MASTL; microtubule associated serine/threonine kinase like; microtubule associated serine/threonine kinase-like; Microtubule-associated serine/threonine-protein kinase-like; RP11-85G18.2; Serine/threonine-protein kinase greatwall; THC2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG