CST, 59735T, GPX4 (E5Y8K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GPX4. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, GPX4 (E5Y8K) Rabbit Monoclonal Antibody (CST #59735) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:1600 - 1:6400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity GPX4 (E5Y8K) Rabbit Monoclonal Antibody recognizes endogenous levels of total GPX4 protein. This antibody is not recommended for immunohistochemical analysis of mouse tissues. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GPX4 protein. Background The selenoprotein glutathione peroxidase 4 (GPX4) is a master regulator of ferroptosis, a form of programmed cell death induced by iron-dependent lipid peroxidation (1,2). GPX4 converts lipid hydroperoxides to non-toxic lipid alcohols, therefore preventing ferroptosis (2). Research studies show that selenium enhances GPX4 expression and inhibits ferroptotic death to protect neurons (3). In addition, some therapy-resistant cancer cells depend on GPX4 to survive. Loss of GPX4 leads to ferroptosis and thus prevents tumor relapse in mice (4). Furthermore, redox homeostasis mediated by GPX4 is essential for activation of the cytosolic DNA-sensing cGAS-STING pathway and initiation of the subsequent innate immune response (5). Alternate Names epididymis secretory sperm binding protein; Glutathione peroxidase 4; glutathione peroxidase 4 (phospholipid hydroperoxidase); GPx-4; GPX4; GSHPx-4; MCSP; PHGPx; phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase, mitochondrial; selenoprotein GPX4; SMDS; snGPx; snPHGPx; sperm nucleus glutathione peroxidase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 20, 22 Source/Isotype: Rabbit IgG