CST, 59817S, Myt1 (F4I2F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Myt1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Myt1 (F4I2F) Rabbit Monoclonal Antibody (CST #59817) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Myt1 (F4I2F) Rabbit Monoclonal Antibody recognizes endogenous levels of total Myt1 protein. Based on the sequence of the protein antigen, this antibody is expected to detect all isoforms of Myt1. This antibody detects approximately 35 kDa and 45 kDa proteins of unknown identity in some cell lines. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human Myt1 protein. Background Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-7). Alternate Names DKFZp547K1610; FLJ20093; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT1; PMYT1; PPP1R126; protein kinase Myt1; protein kinase, membrane associated tyrosine/threonine 1; protein phosphatase 1, regulatory subunit 126 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 60-70 Source/Isotype: Rabbit IgG