CST, 59827SF, ELF3 (F6Z8R) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying ELF3/ESE1b. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, ELF3 (F6Z8R) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #59827) is ready to ship. Product Usage Information This product is the carrier free version of product #31600. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 31600 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity ELF3 (F6Z8R) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total ELF3 protein. Non-specific spotty cytoplasmic staining was observed in a few human tumors by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ELF3 protein. Background The E26 transformation-specific (ETS) transcription factors bind to a core 5'-GGAA/T-3' DNA motif and are known to regulate many biological processes, including cell proliferation, differentiation, apoptosis, hematopoiesis, and angiogenesis; they have also been described in pathological conditions, including cancer (reviewed in 1). ETS transcription factor E74-like factor 3 (ELF3), also called ESE-1, ESX, Jen, and ERT, belongs to a subtype of ETS proteins characterized by a predominant epithelial-specific expression profile (2-6). ELF3 shows widespread expression across tissues with high expression in the digestive tract. Targeted knockout of the gene in mice results in lethality in about 30% of the embryos to E11.5, along with abnormalities in the intestinal epithelium (7). ELF3 can have dualistic roles in cancer with tumor-promoting and suppressing activity (reviewed in 8,9). Dysregulation of ELF3 through genetic and epigenetic mechanisms such as inactivating mutations, promoter methylation, and gene amplification have been reported across multiple cancer types and stages of disease. In addition, ELF3 has been shown to play a role in immune disorders and inflammation (10-12). Alternate Names E74 like ETS transcription factor 3; E74-like factor 3; E74-like factor 3 (ets domain transcription factor, epithelial-specific ); E74-like factor 3 (ETS domain transcription factor, serine box, epithelial-specific); E74-like factor 3 (ets domain transcription factor); ELF3; Epithelial-restricted with serine box; Epithelium-restricted Ets protein ESX; Epithelium-specific Ets transcription factor 1; EPR-1; ERT; ESE-1; ESX; ets domain transcription factor, serine box (epithelial-specific); ETS-related transcription factor Elf-3; JEN Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG