CST, 59854T, Catenin delta-1 (D7S2M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Catenin-delta. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Catenin delta-1 (D7S2M) Rabbit Monoclonal Antibody (CST #59854) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:300 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Catenin delta-1 (D7S2M) Rabbit Monoclonal Antibody recognizes endogenous levels of total catenin δ-1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human catenin δ-1 protein. Background Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4). Alternate Names BCDS2; Cadherin-associated Src substrate; CAS; catenin (cadherin-associated protein), delta 1; catenin delta 1; Catenin delta-1; CTND1; CTNND; CTNND1; KIAA0384; p120; p120 catenin; p120(cas); p120(ctn); P120CAS; P120CTN Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 95, 105 Source/Isotype: Rabbit IgG