CST, 59928S, CD300a Antibody

Polyclonal Antibody for studying CD300a mouse. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, CD300a Antibody (CST #59928) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CD300a Antibody recognizes endogenous levels of total CD300a protein. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse CD300a protein. Antibodies are purified by peptide affinity chromatography. Background CD300a is an inhibitory receptor in the Ig superfamily, a type I transmembrane protein containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs) on its cytoplasmic tail (1,2,5). Human CD300a (CMRF-35H, IRp60) and mouse CD300a (CLM-8, LMIR-1, MAIR-I) are functional orthologs, expressed by monocytes, granulocytes, mast cells, and subsets of B cells (1-5). Human, but not mouse, CD300a is also expressed by unstimulated NK cells and subsets of T cells (1,5). Phosphorylated ITIMs are able to recruit different phosphatases, such as SHP-1 and SHP-2, leading to inhibition of cell activation (1,5). Alternate Names B230315M08Rik; CD300 antigen-like family member A; Cd300a; CD300A antigen; CD300A molecule; Clm; CLM-8; Clm8; CMRF35-like molecule 8; Leukocyte mono-Ig-like receptor 1; Lmir1; MAIR-1; MAIR-I; MAIR-Ia; Mast cell-derived paired immunoglobulin-like receptor 1; Mcpir1; MMAC; MMAC8; Myeloid-associated immunoglobulin-like receptor 1; OTTMUSP00000003633; Pigr4; polymeric immunoglobulin receptor 4 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 40-60 SOURCE: Rabbit