CST, 59964SF, Caspr (D8I3V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Caspr. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Caspr (D8I3V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #59964) is ready to ship. Product Usage Information This product is the carrier free version of product #97736. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 97736 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Caspr (D8I3V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total Caspr protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1375 of human Caspr protein. Background Contactin-associated protein 1 (Caspr) is a membrane protein that is an essential component of the paranodal junctions in the peripheral and central nervous systems (PNS and CNS, respectively). Caspr is part of the Neurexin family of proteins and is also known as Neurexin IV, Paranodin, and Cntnap1. Caspr forms a complex, via its extracellular domain, with contactin at paranodal junctions of the axon (1, 2). Paranodal junctions are specialized junctions in the axon that are formed between the axolemma and the paranodal loops of myelinating glia. Paranodal structures are critical for salutatory conduction in the PNS and CNS. In the absence of Caspr, Caspr knockout mice exhibit mislocalization of other paranodal junction proteins, including contactin and neurofascin (3). Knockout mice also exhibit reduced nerve conduction velocities, as well as behavior defects consistent with abnormal nerve conduction. Therefore, Caspr is a critical component of a protein complex that is likely central to paranodal junction formation and maintenance. Alternate Names CASPR; Caspr1; CHN3; CNTNAP; CNTNAP1; CNTP1; contactin associated protein 1; Contactin-associated protein 1; neurexin 4; Neurexin IV; Neurexin-4; NRXN4; p190 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 190 Source/Isotype: Rabbit IgG