CST, 59973S, HIF-2 alpha (D6T8V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HIF2-alpha. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, HIF-2 alpha (D6T8V) Rabbit Monoclonal Antibody (CST #59973) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μl of antibody and 10 μg of chromatin (approximately 4 × 10^6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity HIF-2 alpha (D6T8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total HIF-2α protein. This antibody does not cross-react with HIF-1α protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly688 of human HIF-2α protein. Background Hypoxia-inducible factor (HIF) is essential for the cellular response to hypoxia (1,2). Under normoxia conditions, the α subunit of HIF is ubiquitinated by von Hippel-Lindau (VHL) protein and is degraded in the ubiquitin/proteasome pathway (1,2). Hypoxia inhibits degradation of the α subunit, which leads to its stabilization (1,2). HIF, in turn, regulates the transcription of a variety of genes that respond to hypoxia conditions (1,2). There are several isoforms of the HIF α subunit (2). Studies have found that HIF-1α and HIF-2α expression is increased in some human cancers (2). HIF-1α has both pro- and anti-proliferative activities, whereas HIF-2α does not possess anti-proliferative activity (2). Therefore, HIF-2α likely plays an important role in tumorigenesis (2,3). Alternate Names Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; Class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain protein 1; Endothelial PAS domain-containing protein 1; EPAS-1; EPAS1; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF-2-alpha; HIF2-alpha; HIF2A; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 Source/Isotype: Rabbit IgG