CST, 59995S, c-Myb (D1B9E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying c-Myb. Validated for Western Blotting,Immunohistochemistry (Paraffin),Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, c-Myb (D1B9E) Rabbit Monoclonal Antibody (CST #59995) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:200 Flow Cytometry (Fixed/Permeabilized): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 49074 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity c-Myb (D1B9E) Rabbit Monoclonal Antibody recognizes endogenous levels of total c-Myb protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human c-Myb protein. Background c-Myb is a transcriptional activator that specifically recognizes the sequence 5'-YAAC[GT]G-3'. It is expressed in hematopoietic progenitor cells, where it plays an important role in the control of proliferation and differentiation (1-3). c-Myb is required for transcription of genes involved in self-renewal of intestinal stem cells. Importantly, c-Myb regulates expression of Lgr5, a protein expressed in putative intestinal stem cells that give rise to all cell lineages of small intestinal crypts (4). c-Myb is reported to be expressed in colon crypt cells and in human colorectal cancer cell lines (5,6). Research has shown that gene translocations and copy number alterations are found in several leukemias, breast cancer, and other solid tumors (7,8). Alternate Names c-myb; c-myb protein (140 AA); c-myb_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; c-myb8B_CDS; Cmyb; efg; MYB; MYB proto-oncogene, transcription factor; oncogene AMV; Proto-oncogene c-Myb; Transcriptional activator Myb; v-myb avian myeloblastosis viral oncogene homolog; v-myb myeloblastosis viral oncogene homolog (avian) Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG