CST, 60005W2, Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) (D5B10) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying Smad5 (Ser463/Ser465) phosphate/Smad1 (Ser463/Ser465) phosphate/Smad9 (Ser465/Ser467) phosphate. Highly specific and rigorously validated in-house, Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) (D5B10) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 647 Conjugate) (CST #60005) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Phospho-SMAD1 (Ser463/465)/ SMAD5 (Ser463/465)/ SMAD9 (Ser465/467) (D5B10) Rabbit mAb (SignalFlex™ Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser463/465 of human SMAD1 and SMAD5 protein. Background Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation, and apoptosis (1,2). BMP receptors are members of the TGF-β superfamily of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation, and activation of these receptors (3-5). They subsequently phosphorylate SMAD1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as SMAD5 and SMAD9 (SMAD8) at their corresponding sites. These phosphorylated SMADs dimerize with the coactivating SMAD4 and translocate to the nucleus, where they regulate the transcription of target genes (5). MAP kinases and CDKs 8 and 9 are also reported to phosphorylate residues in the linker region of SMAD1, including Ser206. Phosphorylation of SMAD1 at Ser206 recruits Smurf1 to the linker region and leads to the degradation of SMAD1 (6). Phosphorylation at this site also promotes SMAD1 transcriptional activity by recruiting YAP to the linker region (7). Alternate Names BSP-1; BSP1; DKFZp781C1895; DKFZp781O1323; Dwfc; hSMAD1; hSmad5; JV4-1; JV41; JV5-1; MAD homolog 1; MAD homolog 5; MAD homolog 9; Mad-related protein 1; MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; MADH1; MADH5; MADH6; MADH9; MADR1; Mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; Mothers against decapentaplegic homolog 9; mothers against decapentaplegic, drosophila, homolog of, 5; Mothers against decapentaplegic, drosophila, homolog of, 9; Mothers against DPP homolog 1; Mothers against DPP homolog 5; Mothers against DPP homolog 9; PPH2; SMA- and MAD-related protein 5; SMAD 1; SMAD 5; SMAD 9; SMAD family member 1; SMAD family member 5; SMAD family member 9; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; SMAD, mothers against DPP homolog 9; SMAD1; SMAD5; SMAD8; SMAD8/9; SMAD8A; SMAD8B; SMAD9; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG