CST, 60040S, KIR2DL3 (D8L3D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KIR2DL3. Validated for Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, KIR2DL3 (D8L3D) Rabbit Monoclonal Antibody (CST #60040) is ready to ship. Product Usage Information Flow Cytometry (Live): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Flow Cytometry (Live) Specificity / Sensitivity KIR2DL3 (D8L3D) Rabbit Monoclonal Antibody recognizes endogenous levels of total KIR2DL3 protein. This antibody weakly cross-reacts with KIR2DL2 proteins in over-expression cell lines. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala173 of human KIR2DL3 protein. Background Killer cell immunoglobulin-like receptors (KIRs) are type 1 transmembrane glycoproteins expressed by natural killer (NK) cells and subsets of CD4, CD8, and γδ T cells (1-5). Analogous to the diversity of their human leukocyte antigen class I (HLA class I) ligands, the KIR genes are polymorphic and the content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (6,7). The KIR proteins are characterized by the number of extracellular immunoglobulin-superfamily domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain (8-10). KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM) (10), while KIR proteins with the short cytoplasmic domain lack an ITIM and instead transduce activating signals (11,12). KIR proteins play an important role in the regulation of the immune response. Combinations of KIR and HLA class I variants influence susceptibility to autoimmunity and infectious disease, as well as outcomes of haematopoietic stem cell transplantation (12-14). KIR2DL3, also referred to as CD158b, interacts with HLA-C alleles (HLA-Cw1, HLA-Cw3, and HLA-Cw7). Upon receptor ligand interaction, KIR2DL3 inhibits the activity of NK cells thus preventing target cell lysis (15-17). Alternate Names CD158 antigen-like family member B2; CD158b; CD158B2; GL183; KI2L3; killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 3; Killer cell immunoglobulin-like receptor 2DL3; killer cell immunoglobulin-like receptor two domains long cytoplasmic tail 3; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 3; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 5; killer inhibitory receptor cl 2-3; KIR-023GB; KIR-K7b; KIR-K7c; KIR2DL; KIR2DL3; KIR2DS5; KIRCL23; MGC129943; MHC class I NK cell receptor; natural killer associated transcript 2; natural killer cell inhibitory receptor KIR2DL3; natural killer-associated transcript 2; Natural killer-associated transcript 2a protein; NK-receptor; NKAT; NKAT-2; NKAT2; NKAT2A; NKAT2a-delta-Ig2; NKAT2B; p58; p58 natural killer cell receptor clone CL-6; p58 NK receptor CL-6; p58.2 MHC class-I specific NK receptor; p58.2 MHC class-I-specific NK receptor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG