CST, 60071S, BDNF (E5H5C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying BDNF. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, BDNF (E5H5C) Rabbit Monoclonal Antibody (CST #60071) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity BDNF (E5H5C) Rabbit Monoclonal Antibody recognizes human and mouse recombinant BDNF, and exogenously expressed BDNF. This antibody detects multiple bands using a purified mature form of the protein, consistent with multimerization of BDNF. This antibody detects both full-length BDNF protein and the mature form of BDNF. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg135 of human BDNF protein. Background Neurotrophins are composed of at least four family members, including NGF, BDNF, NT-3, and NT-4, and all are known to influence growth, development, differentiation, and survival of neurons (1). Proneurotrophins bind to p75NTR, but not to the family of Trk receptor tyrosine kinases (Trk), and following maturation, BDNF binds and activates TrkB. Trk receptors, in turn, activate three major signaling pathways: (a) Ras-MAPK signaling, which promotes neuronal differentiation and neurite outgrowth, (b) PI3K/Akt signaling, which promotes survival and growth of neurons, and (c) PLC-γ1-PKC signaling, which promotes synaptic plasticity (2). BDNF is a major regulator of transmission and plasticity at adult synapses. Moreover, the precursor proBDNF and the mature protein mBDNF drive opposite effects on long-term potentiation and long-term depression (3). BDNF has also been implicated in body weight regulation and activity: heterozygous BDNF knockout mice are hyperphagic, obese, and hyperactive (4). Alternate Names Abrineurin; ANON2; BDNF; BDNF precursor form; brain derived neurotrophic factor; Brain-derived neurotrophic factor; BULN2; MGC34632; neurotrophin; ProBDNF Specification REACTIVITY: H M SENSITIVITY: Recombinant and Transfected MW (kDa): ProBDNF 28 - Mature BDNF 12-14 Source/Isotype: Rabbit IgG