CST, 60133T, GAMT (F8E4G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GAMT. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, GAMT (F8E4G) Rabbit Monoclonal Antibody (CST #60133) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity GAMT (F8E4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total GAMT protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GAMT protein. Background Guanidinoacetate N-methyltransferase (GAMT) is an enzyme member of the transferase protein family that functions in creatine synthesis. Creatine is a naturally occurring compound that plays a role in maintaining ATP levels within metabolically active tissues such as muscle and brain. While creatine is mainly synthesized in the kidneys and liver, it is also endogenously synthesized in the brain (1,2). Creatine synthesis occurs through the conversion of arginine and glycine to guanidinoacetate (GAA) by arginine:glycine amidinotransferase (AGAT). GAA is then methylated by GAMT, forming creatine (3). In the brain, this system is essential to maintaining the development and function of the central nervous system, with deficiency in either GAMT or AGAT leading to neurological consequences. GAMT deficiency is a disorder caused by mutations in the gene, which disrupts the creatine synthesis pathway, leading to developmental delays, varying intellectual disability, and seizure disorders (4,5). Alternate Names CCDS2; epididymis secretory protein Li 20; GAMT; Guanidinoacetate N-methyltransferase; HEL-S-20; PIG2; TP53I2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG