CST, 60153S, NDUFS1 Antibody

Polyclonal Antibody for studying NDUFS1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, NDUFS1 Antibody (CST #60153) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NDUFS1 Antibody recognizes endogenous levels of total NDUFS1 protein. This antibody can detect the carboxyl terminal 47 kDa fragment produced by caspase cleavage during apoptosis. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile286 of human NDUFS1 protein. Antibodies are purified by peptide affinity chromatography. Background NDUFS1 (NADH dehydrogenase Fe-S protein 1) is a nuclear encoded structural subunit of NADH: ubiquinone oxidoreductase (complex 1) in the mitochondrial electron transport chain (1). Mutations in NDUFS1 and other complex 1 subunits leading to mitochondrial dysfunction are associated with a number of neurological disorders (2-5). High-fat diet associated with type 2 diabetes leads to decreased expression of complex 1 subunits, including NDUFS1 (6). NDUFS1 is the target of cleavage by apoptotic caspases contributing to the loss of mitochondrial transmembrane potential, compromised mitochondrial respiration, increased mitochondrial reactive oxygen species (ROS) production, and loss of lysosomal integrity (7,8). NDUFS1 can also be cleaved by the lymphocyte protease granzyme B (9). Studies have also found that MDM2, which has a canonical role as an inhibitor of the tumor suppressor p53, can also sequester NDUFS1 in the cytoplasm, leading to respiratory dysfunction and increases in ROS (10). Alternate Names CI-75k; CI-75kD; complex I 75kDa subunit; Complex I-75kD; complex I, mitochondrial respiratory chain, 75-kD subunit; MC1DN5; MGC26839; mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit; NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase); NADH-coenzyme Q reductase; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial; NADH:ubiquinone oxidoreductase core subunit S1; NDUFS1; NDUS1; PRO1304 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 75 SOURCE: Rabbit