CST, 60175W2, E-Cadherin (24E10) Rabbit Monoclonal Antibody (SignalFlex™ mFluor™ UV375 Conjugate)

Monoclonal Antibody for studying E-Cadherin. Highly specific and rigorously validated in-house, E-Cadherin (24E10) Rabbit Monoclonal Antibody (SignalFlex^™ mFluor^™ UV375 Conjugate) (CST #60175) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity E-Cadherin (24E10) Rabbit mAb (SignalFlex™ mFluor™ UV375 Conjugate) detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein. Background Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). Alternate Names Arc-1; BCDS1; CADH1; Cadherin; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); Cadherin E; Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324; CDH1; CDHE; cell-CAM 120/80; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; E-cadherin; E-cadherin 1; ECAD; epididymis secretory sperm binding protein; Epithelial cadherin; LCAM; UVO; Uvomorulin Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG