CST, 60322S, IDH2 (KrMab-3) Mouse Monoclonal Antibody

Monoclonal Antibody for studying IDH2. Validated for WB,IP,IHC,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, IDH2 (KrMab-3) Mouse Monoclonal Antibody (CST #60322) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:50 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity IDH2 (KrMab-3) Mouse Monoclonal Antibody recognizes endogenous levels of total IDH2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala174 of human IDH2 protein, as described by Kaneko et al. (2013) [8]. Background IDH2 is one of three isocitrate dehydrogenases (IDH1-3) that catalyze the oxidative decarboxylation of isocitrate to produce CO and α-ketoglutarate (α-KG). These enzymes belong to two distinct subclasses that utilize either NAD or NADP as an electron acceptor. IDH2 is an NADP -dependent isocitrate dehydrogenase expressed primarily in the mitochondria, where it also functions in the TCA cycle (1,2). Mutations in IDH2 or its cytoplasmic counterpart (IDH1) have been reported in glioblastoma multiforme (3), acute myeloid leukemia (4,5), and other malignancies (6). Research studies have shown that gain-of-function mutations in IDH2 can lead to the accumulation and secretion of the oncometabolite R-2-hydroxyglutarate (2HG) in cancer cells (6,7). Alternate Names D2HGA2; ICD-M; IDH; IDH2; IDHM; IDHP; IDP; IDPM; isocitrate dehydrogenase (NADP(+)) 2; isocitrate dehydrogenase (NADP(+)) 2, mitochondrial; Isocitrate dehydrogenase [NADP], mitochondrial; isocitrate dehydrogenase 2 (NADP+), mitochondrial; isocitrate dehydrogenase, mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; NADP+-specific ICDH; Oxalosuccinate decarboxylase Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Mouse IgG2b