CST, 60327S, beta-crystallin B1/crybb1 (F6Y9L) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CRYBB1 mouse. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, beta-crystallin B1/crybb1 (F6Y9L) Rabbit Monoclonal Antibody (CST #60327) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity beta-crystallin B1/crybb1 (F6Y9L) Rabbit Monoclonal Antibody recognizes endogenous levels of total β-crystallin B1 protein. Non-specific nuclear staining was observed in many normal mouse tissues by immunohistochemistry. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse β-crystallin B1 protein. Background β-crystallin B1, encoded by , is a member of the β-crystallins, one of three major crystallin types encoded by the mammalian genome, which also includes alpha- and gamma-crystallins. Crystallins are expressed at very high levels in the retinal lens and form homo/heterodimers via its N-terminal arms (1). Crystallins generally function to maintain the transparency and refractive power of the eye lens. However, they are expressed outside of the lens in other tissues and have alternative non-lens functions (2). For example, Nuc1 mutant rat, in which the β gene is mutated, exhibit vascular remodeling abnormalities via an astrocyte-dependent mechanism (3,4). The precise cellular function of β-crystallins in glia is not well understood but may involve regulation of endolysosomal acidification, Golgi trafficking, and specific forms of cell death (5,6). Interestingly, is highly enriched in microglia outside of the mammalian lens and may serve as a distinct marker for microglia (7). Alternate Names 3110006K12Rik; BB1CRY; Beta-B1 crystallin; Beta-crystallin B1; Beta-crystallin B1B; CRBB1; Crybb1; crystallin, beta B1; OTTMUSP00000029911; OTTMUSP00000029913 Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Rabbit IgG