CST, 60356S, Nanog (D2A3) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying Nanog mouse. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Nanog (D2A3) Rabbit Monoclonal Antibody (PE Conjugate) (CST #60356) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Nanog (D2A3) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total mouse nanog protein. This antibody conjugate is expected to recognize nanog 1a and 1b isoforms. This antibody conjugate does not cross-react with human nanog. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse nanog protein. Background Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28 (2,3). Alternate Names 2410002E02Rik; early embryo specific expression NK family; Early embryo specific expression NK-type homeobox protein; ecat; ecat4; ENK; ES cell-associated protein 4; Homeobox protein NANOG; homeobox transcription factor Nanog; Nanog; Nanog homeobox; Q5TM83; Q80Z64; Q80Z64-2 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG