CST, 60386T, RBM15 (E8Y8A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RBM15. Validated for Western Blotting,Immunoprecipitation,eCLIP. Available in 2 sizes. Highly specific and rigorously validated in-house, RBM15 (E8Y8A) Rabbit Monoclonal Antibody (CST #60386) is ready to ship. Product Usage Information For more information about the RBP-eCLIP service please visit Eclipsebio . Western Blotting: 1:1000 Immunoprecipitation: 1:50 eCLIP: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RBM15 (E8Y8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total RBM15 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala557 of human RBM15 protein. Background RBM15 is an RNA-binding protein that is part of the WTAP-METTL3 m6A methyltransferase complex. RBM15 and related RBM15B interact with WTAP to recruit the complex to target mRNAs, and are critical to XIST-mediated gene silencing (1). RBM15 can recruit splicing factors such as SF3B1 to mRNA to promote alternative splicing. Expression levels of RBM15 can be regulated by PRMT1, which can methylate R578, resulting in RBM15 ubiquitinylation and degradation (2). This process is critical in acute megakaryoblastic leukemia, a cancer type where RBM15 is fused to the gene and PRMT1 is overexpressed (3). RBM15 normally plays roles in hematopoietic development and myeloid differentiation, where it can regulate the levels of c-Myc (4-6). Alternate Names FLJ12479; FLJ21943; MGC119584; one twenty two protein; one twenty-two; One-twenty two protein 1; OTT; OTT1; Putative RNA-binding protein 15; RBM15; RNA binding motif protein 15; RNA-binding motif protein 15; RNA-binding protein 15; SPEN Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG