CST, 60406S, MGEA5/OGA (E9C5U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OGA. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, MGEA5/OGA (E9C5U) Rabbit Monoclonal Antibody (CST #60406) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MGEA5/OGA (E9C5U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MGEA5/OGA protein. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys545 of human MGEA5/OGA protein. Background O-GlcNAcylation is a post-translational modification where β-D-N-acetylglucosamine (GlcNAc) is covalently linked to cytoplasmic and nuclear proteins at serine or threonine residues (1,2). This modification is important in many cellular processes, including metabolism, cell growth and morphogenesis, apoptosis, and transcription (2,3). The reversible protein modification by O-GlcNAc, which has been suggested to be a nutrient and stress sensor, is catalyzed by two highly conserved enzymes, O-GlcNAc transferase (OGT) and human meningioma-expressed antigen 5 (MGEA5)/O-GlcNAcase (OGA) (4). Research studies have implicated O-GlcNAcylation in cancer (1). This modification has also been proposed to have protective effects against the production and aggregation of toxic protein species associated with neurodegenerative diseases, including amyloid β (Aβ) and tau (Alzheimer's disease), and α-synuclein (Parkinson's disease) (5-7).  Alternate Names Beta-hexosaminidase; Beta-N-acetylglucosaminidase; Beta-N-acetylhexosaminidase; Bifunctional protein NCOAT; FLJ11229; FLJ23355; HAT; HEXC; Hexosaminidase C; Histone acetyltransferase; hyaluronidase in meningioma; KIAA0679; MEA5; meningioma expressed antigen 5 (hyaluronidase); Meningioma-expressed antigen 5; MGEA5; N-acetyl-beta-D-glucosaminidase; N-acetyl-beta-glucosaminidase; NCOAT; Nuclear cytoplasmic O-GlcNAcase and acetyltransferase; O-GlcNAcase; OGA; Protein O-GlcNAcase Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 138 Source/Isotype: Rabbit IgG