CST, 60432S, Fc-gamma RIIA (E3K8U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FcGR2A. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Fc-gamma RIIA (E3K8U) Rabbit Monoclonal Antibody (CST #60432) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Fc-gamma RIIA (E3K8U) Rabbit Monoclonal Antibody recognizes endogenous levels of total FcγRIIA protein. This antibody does not cross-react with FcγRIIB protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro170 of human FcγRIIA protein. Background FcγRIIA (CD32A) is a low affinity, IgG Fc-binding receptor belonging to the immunoglobulin receptor superfamily (1-3). In humans, it is expressed on myeloid cells, dendritic cells, and platelets (2-4). FcγRIIA is an activating-type Fc receptor, and signals through an immunoreceptor tyrosine-based activation motif (ITAM) present on its own IgG binding chain (2-4). ITAM signaling is essential for FcγRIIA-dependent phagocytosis and the induction of cytokine secretion (3). In addition to binding to IgG, FcγRI and FcγRII also bind to pentraxin proteins of the innate immune system (5). Alternate Names CD32; CD32A; CDw32; Fc fragment of IgG receptor IIa; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc gamma receptor RIIa3; Fc-gamma RII-a; Fc-gamma-RIIa; FCG2; FCG2A; FcGR; FCGR2; FCGR2A; FCGR2A1; FcRII-a; IGFR2; IgG Fc receptor II-a; Immunoglobulin G Fc receptor II; Low affinity immunoglobulin gamma Fc region receptor II-a; MGC23887; MGC30032 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG