CST, 60433T, Pax6 (D3A9V) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PAX6. Validated for WB,IP,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Pax6 (D3A9V) Rabbit Monoclonal Antibody (CST #60433) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:250 - 1:1000 Immunofluorescence (Frozen): 1:3200 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Pax6 (D3A9V) Rabbit Monoclonal Antibody recognizes endogenous levels of total Pax6 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Pax6 protein. Background Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2). Pax6 has important functions in organ development. It is a key regulator of eye development (5), and mutations in Pax6 have been associated with some forms of aniridia, a congenital malformation of the eye (6). Pax6 is also involved in neuronal development, which plays an especially important role in corticogenesis (7). Within its role in the adult brain, it has recently been associated with aging, with gene occupancy studies showing increased association of Pax6 with genes associated with many aging processes in mice (8,9). Pax6 also plays another important function in development and maintenance of pancreatic β-cells (10). Driven by expression of Pdx1, both Pax6 and NGN3 expression is required for β-cell identity (11). Alternate Names AN; AN1; AN2; Aniridia 1; Aniridia 2; Aniridia type II protein; ASGD5; D11S812E; FVH1; MGC17209; MGDA; Oculorhombin; paired box 6; paired box homeotic gene-6; Paired box protein Pax-6; PAX6; WAGR Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG