CST, 60480SF, p21 Waf1/Cip1 (DCS60) Mouse Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying p21 Waf1/Cip1. Validated for Western Blotting,Immunohistochemistry (Paraffin),ELISA+. Highly specific and rigorously validated in-house, p21 Waf1/Cip1 (DCS60) Mouse Monoclonal Antibody (BSA and Azide Free) (CST #60480) is ready to ship. Product Usage Information This product is the carrier free version of product #2946. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2946 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity p21 Waf1/Cip1 (DCS60) Mouse Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total p21 protein. This antibody does not cross-react with other cdk inhibitors. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human p21 corresponding to the amino-terminal portion of p21. Background The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5). Alternate Names CAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CDKN1A; CDN1A; CIP1; cyclin dependent kinase inhibitor 1A; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; MDA6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1; WAF1; wild-type p53-activated fragment 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 21 Source/Isotype: Mouse IgG2a