CST, 60487S, Lysozyme C-1/2 (E2K5R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying LYZ2 mouse/LYZ1 mouse. Validated for WB,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Lysozyme C-1/2 (E2K5R) Rabbit Monoclonal Antibody (CST #60487) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:1600 - 1:6400 Immunofluorescence (Frozen): 1:400 - 1:1600 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Lysozyme C-1/2 (E2K5R) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse and rat Lysozyme C-1 and Lysozyme C-2 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu139 of mouse Lysozyme C-1/2 protein. Background Lysozymes are secreted proteins that have bacteriolytic function which are critical for mammalian innate immune function. All lysozymes function to defend host animals from microbial infection by the hydrolysis of bacterial cell wall peptidoglycan (1). Conventional-type lysozymes (Lysozyme C) are one of three types of lysozymes; each family member is categorized based on amino acid sequence and biochemical properties. Lysozyme C is expressed in mammalian secretions like tears, urine, and milk but is also expressed by phagocytes such as macrophages, neutrophils, and dendritic cells. Lysozyme C is encoded in humans by a single gene. The mouse orthologs of Lysozyme C are encoded by two genes, and , which encode Lysozyme C-1 and Lysozyme C-2 (Lysozyme C-1/2). Interestingly, is upregulated in microglia of Alzheimer's disease mouse model brains that have been stimulated by specific forms of activity (2). and are uniquely expressed in microglia, and increased correlates with microglia-mediated β-amyloid (Aβ) clearance, suggesting that Lysozyme C-1/2 may directly contribute to microglial-clearance of Aβ or act as a marker for certain microglial activity states in the brain (3). Alternate Names 1,4-beta-N-acetylmuramidase C; AI326280; Lys; Lysm; lysozyme 1; lysozyme 2; Lysozyme C type M; Lysozyme C type P; Lysozyme C-1; Lysozyme C-2; lysozyme f2; lysozyme f3; Lyz; Lyz1; Lyz2; Lyzf2; Lyzf3; Lyzs; Lzm; Lzm-; Lzm-s1; Lzp; Lzp-s; P lysozyme structural; renal amyloidosis Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 17 Source/Isotype: Rabbit IgG