CST, 60538S, Histone Deacetylase 3 (HDAC3) Antibody

Polyclonal Antibody for studying HDAC3. Validated for Western Blotting. Highly specific and rigorously validated in-house, Histone Deacetylase 3 (HDAC3) Antibody (CST #60538) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Histone Deacetylase 3 (HDAC3) Antibody detects endogenous levels of total HDAC3 protein. This antibody does not cross-react with other HDAC proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human HDAC3. Antibodies are purified by protein A and peptide affinity chromatography. Background Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). Alternate Names HD3; HDAC3; Histone deacetylase 3; KDAC3; RPD3; RPD3-2; SMAP45 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 49 SOURCE: Rabbit