CST, 60712S, CD86/B7-2 (GL-1) Rat Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying CD86 mouse. Validated for Flow Cytometry (Fixed/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, CD86/B7-2 (GL-1) Rat Monoclonal Antibody (PE Conjugate) (CST #60712) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.125 μg of antibody per test. Flow Cytometry (Fixed/Permeabilized): 1:160 Flow Cytometry (Live): 1:160 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized), Flow Cytometry (Live) Specificity / Sensitivity CD86/B7-2 (GL-1) Rat Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of total CD86/B7-2 protein. This antibody conjugate detects an epitope within the extracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background CD80 (B7-1, BB1) and CD86 (B7-2, B70) are members of the B7 family of cell surface ligands that regulate T cell activation and immune responses. CD80 is expressed on activated antigen presenting cells, including dendritic cells, B cells, monocytes, and macrophages. CD86 is expressed on resting monocytes, dendritic cells, activated B lymphocytes, and can be further upregulated in the presence of inflammation (1-3). CD80 and CD86 are ligands for CD28, which functions as a T cell costimulatory receptor. Interaction of CD28 with CD80 or CD86 provides the second signal required for naïve T cell activation, T cell proliferation, and acquisition of effector functions (3-7). Alternatively, CD80 and CD86 also act as ligands to CTLA-4, which results in the downregulation of T cell activity (3,7-9). The GL-1 antibody is used as a marker for CD86 expression on B cells, macrophages, and dendritic cells. Alternate Names Activation B7-2 antigen; B7; B7-2; B7.2; B70; Cd28l; Cd28l2; Cd86; CD86 antigen; CLS1; early T cell costimulatory molecule-1; Early T-cell costimulatory molecule 1; ETC-1; Ly-5; Ly-58; Ly5; Ly58; MB7; MB7-2; OTTMUSP00000015940; T-lymphocyte activation antigen CD86; TS/A-2 Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Rat IgG2a kappa