CST, 60732T, NDP52 (D1E4A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NDP52. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NDP52 (D1E4A) Rabbit Monoclonal Antibody (CST #60732) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NDP52 (D1E4A) Rabbit Monoclonal Antibody recognizes endogenous levels of total NDP52 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val135 of human NDP52 protein. Background NDP52 (CALCOCO2) is ubiquitously expressed and composed of an amino-terminal SKICH domain, followed by a coiled-coil domain, and two zinc finger domains (1). It has recently been reported to act as an autophagy receptor that binds cytosolic ubiquitinated bacteria, leading to autophagy activation and pathogen clearance (1-3). NDP52 binds ubiquitin through its zinc finger domain and simultaneously binds LC3, which directs the bacteria into autophagosomes. In addition, NDP52 interacts with Nap1 and SINTBAD to recruit TBK1 to ubiquitinated bacteria (1). Alternate Names Antigen nuclear dot 52 kDa protein; CACO2; calcium binding and coiled-coil domain 2; Calcium-binding and coiled-coil domain-containing protein 2; CALCOCO2; MGC17318; NDP52; Nuclear domain 10 protein 52; Nuclear domain 10 protein NDP52; Nuclear dot protein 52 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 52, 60 Source/Isotype: Rabbit IgG