CST, 60758S, NAF-1/CISD2 (D4X4E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CISD2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NAF-1/CISD2 (D4X4E) Rabbit Monoclonal Antibody (CST #60758) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NAF-1/CISD2 (D4X4E) Rabbit Monoclonal Antibody recognizes endogenous levels of total NAF-1/CISD2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu70 of human NAF-1/CISD2 protein. Background Nutrient-deprivation autophagy factor-1 (NAF-1) (also known as CISD2, CDGSH iron-sulfur domain-containing protein 2) is a member of NEET family of 2Fe-2S proteins, characterized by a unique CDGSH sequence at their Fe-S-cluster-binding domain (1). NAF-1/CISD2 is a multifunctional protein. In addition to its role in iron and ROS homeostasis, it has been shown to play a role in autophagy, neurodegenerative diseases, and aging (1-6). Enhanced expression of NAF-1/CISD2 is associated with many types of cancer. Silencing of NAF-1/CISD2 expression in cancer cells significantly inhibited proliferation and tumorigenicity; while overexpression of NAF-1/CISD2 significantly enhanced proliferation (2,7,8). Alternate Names CDGSH iron sulfur domain 2; CDGSH iron sulfur domain-containing protein 2; CDGSH iron-sulfur domain-containing protein 2; CDGSH2; CISD2; Endoplasmic reticulum intermembrane small protein; ERIS; Miner1; mitoNEET related 1; MitoNEET-related 1 protein; NAF-1; Nutrient-deprivation autophagy factor-1; WFS2; ZCD2; zinc finger, CDGSH-type domain 2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 15 Source/Isotype: Rabbit IgG