CST, 60769S, Importin beta1 (E1F1G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Importin beta-1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Importin beta1 (E1F1G) Rabbit Monoclonal Antibody (CST #60769) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Importin beta1 (E1F1G) Rabbit Monoclonal Antibody recognizes endogenous levels of total importin β1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn486 of human importin β1 protein. Background Importins belong to the karyopherin family of nuclear transport proteins (1) and are divided into two subgroups: importin α and importin β. Importins mainly function in nuclear protein import and export (2,3). Importin β1 (also known as karyopherin β1, Kpnβ1, Kpnb1, or p97) plays a key role in the nuclear import process (1-3). Nuclear import via importin β1 association with adaptor importin α (also known as karyopherin α, or Kpnα) is an essential component of the classical nuclear localization signal (NLS) pathway (4). Importin α directly recognizes the NLS present in the cargo target, prompting complex formation with importin β1. The cargo:importin α:importin β1 complex is transported across the nuclear pore complex (NPC) into the nucleus, where it is dissociated by the binding of RanGTP (1-4). Nuclear import directly via importin β1 can also occur by importin β1 recognition of the cargo protein, bypassing importin α involvement. In both cases, the importin β1/target protein interaction is mediated through the binding of importin β1 HEAT repeats with the target protein sequences (either the cargo protein itself or importin α) (5). Alternate Names IMB1; Impnb; importin 1; importin 90; importin beta-1 subunit; Importin subunit beta-1; Importin-90; IPO1; IPOB; karyopherin (importin) beta 1; karyopherin subunit beta 1; Karyopherin subunit beta-1; KPNB1; MGC2155; MGC2156; MGC2157; NTF97; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 97 Source/Isotype: Rabbit IgG