CST, 60774S, TCF12/HEB (E8R5E) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying TCF12/HEB. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, TCF12/HEB (E8R5E) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #60774) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity TCF12/HEB (E8R5E) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Trp60 of human TCF12/HEB protein. Background TCF12/HEB (T cell factor 12/HeLa E-box binding protein) is a member of the E proteins that are type I basic helix-loop-helix domain-containing transcription factors that bind directly to the E-box DNA consensus sequence and control numerous developmental processes (1,2). E protein family members include E2A, E2-2, and TCF12/HEB. TCF12/HEB forms homo- or heterodimers with other E proteins to regulate transcription of target genes that play a critical role in the development of T cells, B cells, and plasmacytoid dendritic cells (2-4). The gene has two transcriptional start sites leading to the generation of the long form called HEBCan, and the short form, HEBAlt (5). The two isoforms are thought to have distinct functions, with HEBCan playing a role throughout T cell development, while HEBAlt functions in the efficient generation of T cell precursors (5). Alternate Names bHLHb20; class B basic helix-loop-helix protein 20; CRS3; DNA-binding protein HTF4; E-box-binding protein; HEB; helix-loop-helix transcription factor 4; Helix-loop-helix transcription factor 4 isoform c; HsT17266; HTF4; p64; TCF-12; TCF12; Transcription factor 12; Transcription factor 12 (HTF4, helix-loop-helix transcription factors 4), isoform CRA_b; transcription factor HTF-4 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG