CST, 60786SF, UCP1 (E9Z2V) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying UCP1. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, UCP1 (E9Z2V) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #60786) is ready to ship. Product Usage Information This product is the carrier free version of product #72298. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 72298 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity UCP1 (E9Z2V) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total UCP1 protein. This antibody does not cross-react with UCP2 or UCP3 protein. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro152 of human UCP1 protein. Background Uncoupling protein 1 (UCP1) is a mitochondrial inner membrane transport protein that is primarily expressed in brown adipose tissue (BAT). UCP1 dissipates the pH gradient resulting from oxidative phosphorylation, which uncouples ATP synthesis from oxidative phosphorylation and leads to the release of heat energy. As a result, UCP1 plays an important role in thermogenesis (reviewed in 1). Research studies indicate that subcutaneous white adipose depots in mice contain beige adipocytes that express low levels of UCP1 protein (2). Additional studies show possible differences in thermogenesis in individuals carrying specific polymorphisms in the corresponding gene (3). Related studies link UCP1 to the possible development of obesity and type 2 diabetes (4). Alternate Names Mitochondrial brown fat uncoupling protein 1; SLC25A7; Solute carrier family 25 member 7; Thermogenin; UCP; UCP 1; UCP1; uncoupling protein 1; uncoupling protein 1 (mitochondrial, proton carrier) Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 30 Source/Isotype: Rabbit IgG