CST, 60796S, DGK alpha (E7T8H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying DGKA. Validated for Western Blotting,Immunoprecipitation,Flow Cytometry (Fixed/Permeabilized). Available in 2 sizes. Highly specific and rigorously validated in-house, DGK alpha (E7T8H) Rabbit Monoclonal Antibody (CST #60796) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Flow Cytometry (Fixed/Permeabilized): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity DGK alpha (E7T8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total DGKα ​​​​​​​ protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu734 of human DGKα protein. Background Diacylglycerol kinase alpha (DGKA) is a type 1 diacylglycerol kinase that competes with protein kinase C to convert messenger diacylglycerol (DAG) to phosphatidic acid (1). Upon activation of this intracellular lipid kinase, DGKA rapidly relocates from the cytosol to the plasma membrane where it is involved in multiple cellular processes, including regulating lipid and glucose metabolism levels, cell growth, and cell signaling (2,3). Expression of DGKA is high in T lymphocytes, and detectable in endothelial cells, epithelial cells, fibroblasts, and oligodendrocytes (4). DGKA negatively regulates T cell receptor-dependent responses through phosphorylation of phospholipase C-derived DAG (5-7). In contrast, DGKA-dependent phosphatidic acid generation by phosphorylation of a pre-existing DAG pool is necessary for IL-2-dependent T cell proliferation (8). DGKA is frequently overexpressed in cancer, promoting tumor cell survival, proliferation, invasion, and metastasis (9-12). Due to its role in immunity and cancer, targeting DGKA represents a unique opportunity for therapeutic intervention in cancer (13). Alternate Names 80 kDa diacylglycerol kinase; DAG kinase alpha; DAGK; DAGK1; DGK-alpha; DGKA; Diacylglycerol kinase alpha; diacylglycerol kinase, alpha 80kDa; Diglyceride kinase alpha; MGC12821; MGC42356 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG