CST, 60859S, SLAMF6/CD352 (E3H1E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SLAMF6. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, SLAMF6/CD352 (E3H1E) Rabbit Monoclonal Antibody (CST #60859) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product # 53988 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity SLAMF6/CD352 (E3H1E) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLAMF6/CD352 protein. This antibody does not cross-react with SLAMF3/Ly9, SLAMF4/2B4, or SLAMF5/CD84 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SLAMF6/CD352 protein. Background SLAMF6 (CD352/NTB-A) is a type-I transmembrane glycoprotein belonging to the signaling lymphocytic activation molecule (SLAM) family of immunomodulatory receptors. Like other members of the SLAM receptor family, SLAMF6 contains Ig-like domains within its extracellular region and conserved tyrosine-based signaling motifs within its intracellular domain that, when phosphorylated, bind to the SAP and EAT-2 signaling adaptors (1). SLAMF6 is expressed on the surface of multiple types of immune cells, such as those of the B, T, and NK lineages. Its activation is triggered by homotypic interactions involving its extracellular domain (1-3). Indeed, research studies have shown that in T cells, SLAMF6 engagement facilitates activation and cytokine production (4). Similarly, homotypic ligand-mediated engagement of SLAMF6 on NK cells activates signaling cascades that drive proliferation, cytotoxicity, and cytokine production (1,5-7). Alternate Names Activating NK receptor; CD352; KALI; KALIb; Ly108; MGC104953; natural killer-, T- and B-cell antigen; NK-T-B-antigen; NTB-A; NTBA; NTBA receptor; SF2000; SLAF6; SLAM family member 6; SLAMF6 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 60 Source/Isotype: Rabbit IgG