CST, 60866T, XRN1 (F1Z6T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying XRN1. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, XRN1 (F1Z6T) Rabbit Monoclonal Antibody (CST #60866) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity XRN1 (F1Z6T) Rabbit Monoclonal Antibody recognizes endogenous levels of total XRN1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1397 of human XRN1 protein. Background 5'-3' exoribonuclease 1 (XRN1) is a cytoplasmic exonuclease that degrades RNA containing a 5'-monophosphate to component mononucleotides. XRN1 is the primary exonuclease associated with ribosomes in the cytoplasm and is responsible for mRNA turnover (1,2). This turnover is facilitated in discrete structures in the cytoplasm called P-bodies that contain decapping and deadenylation proteins (3). XRN1 also plays a role in RISC-mediated mRNA degradation, as it associates with 3' mRNA fragments generated by RISC cleavage. This process does not require decapping or deadenylation (4). XRN1 plays a significant role in viral RNA degradation (5). As such, many viral genomes, including hepatitis C, Dengue, and West Nile, encode for XRN1-resistant long non-coding RNA that affects innate immunity and viral replication (6). Alternate Names 5'-3' exoribonuclease 1; DKFZp434P0721; DKFZp686B22225; DKFZp686F19113; FLJ41903; SEP1; Strand-exchange protein 1 homolog; XRN1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 200 Source/Isotype: Rabbit IgG