CST, 60876SF, NKX2.5 (E1Y8H) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying NKX2-5. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, NKX2.5 (E1Y8H) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #60876) is ready to ship. Product Usage Information This product is the carrier free version of product #8792. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 8792 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity NKX2.5 (E1Y8H) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total NKX2.5 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro67 of human NKX2.5 protein. Background NKX2.5 is a member of the NKX homeobox transcription factor family. NKX2.5 plays an essential role in heart development and is among the earliest factors expressed in the cardiac lineage in developing embryos. Targeted disruption of the murine gene results in abnormal heart morphogenesis, severe growth retardation, and embryonic lethality around E9.5 (1,2). Mutations in are likewise associated with several congenital heart conditions, such as atrial defect with atrioventricular conduction defects (ASD-AVCD) and Tetralogy of Fallot (TOF) (3,4). Transcriptional activation of is also associated with some B and T cell leukemias that result from chromosomal translocation (5-8). Alternate Names Cardiac-specific homeobox; cardiac-specific homeobox 1; CHNG5; CSX; CSX1; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; HLHS2; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein NKX 2-5; Homeobox protein Nkx-2.5; NK2 homeobox 5; NK2 transcription factor related, locus 5; NK2 transcription factor related, locus 5 (Drosophila); NKX 2-5; NKX2-5; NKX2.5; NKX25; NKX2E; NKX4-1; tinman homolog; tinman paralog; VSD3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 30-42 Source/Isotype: Rabbit IgG