CST, 60896S, Integrin alphaV (D2N5H) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Integrin Alpha V. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Integrin alphaV (D2N5H) Rabbit Monoclonal Antibody (CST #60896) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Integrin alphaV (D2N5H) Rabbit Monoclonal Antibody recognizes endogenous levels of total Integrin αV. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human integrin αV protein. Background Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and eight β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2). Several αV subfamily members, including αVβ3, αVβ5, and αVβ1, are highly expressed in active endothelial cells and cancer cells (3-6), where they play a critical role in angiogenesis and tumor metastasis (7-9). Therefore, interest has focused on αV integrin as a key therapeutic target in cancer treatment (10-12). Alternate Names antigen identified by monoclonal antibody L230; CD51; DKFZp686A08142; Integrin alpha-V; Integrin alpha-V heavy chain; Integrin alpha-V light chain; integrin alphaVbeta3; integrin subunit alpha V; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); ITAV; ITGAV; MSK8; Vitronectin receptor; Vitronectin receptor subunit alpha; VNRA; VTNR Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 140, 135 Source/Isotype: Rabbit IgG