CST, 60940S, Astrin/SPAG5 Antibody

Polyclonal Antibody for studying Astrin/SPAG5. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Astrin/SPAG5 Antibody (CST #60940) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Astrin/SPAG5 Antibody recognizes endogenous levels of total Astrin/SPAG5 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val185 of human Astrin/SPAG5 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Astrin/SPAG5 was identified as a microtubule-associated protein in a mitotic extract (1). It is essential for cells to assemble biploar spindle structures and progress through mitosis (1, 2). Astrin/SPAG5 was also identified to be a component associated with outer dense fibers in the sperm tail (3). In addition, this protein negatively regulates mTORC1 activity during the cell stress response (4). Under stress conditions, Astrin/SPAG5 interacts with the mTORC1 component raptor and recruits raptor to stress granules, thereby suppressing mTORC1 formation (4). The inhibition of mTORC1 prevents its hyperactivation and thus keeps cells from undergoing apoptosis during stresses (4). Furthermore, Astrin/SPAG5 has been implicated to be a prognostic marker in breast cancer (5). Alternate Names Astrin; Deepest; hMAP126; MAP126; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; Mitotic spindle-associated protein p126; SPAG5; sperm associated antigen 5; Sperm-associated antigen 5 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 135, 150 SOURCE: Rabbit