CST, 60968S, ZBP1 Antibody

Polyclonal Antibody for studying ZBP1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, ZBP1 Antibody (CST #60968) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ZBP1 Antibody recognizes endogenous levels of total ZBP1 protein. A band of unknown identity is detected at around 19 kDa. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln372 of human ZBP1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background ZBP1 (Z-DNA binding protein 1), also referred to as DAI (DNA-dependent activator of IFN-regulatory factors) and DLM-1, is a nucleotide binding protein that plays a role in tumorigenesis and innate immune responses to viral infection (1). It is expressed at high levels in lymphatic tissues and intestine and is induced in macrophages by interferon gamma or by LPS (2,3). It contains two amino-terminal Z-alpha domains that bind to left-handed Z-DNA and Z-RNA (4,5). In addition, an adjacent domain binds right-handed B-DNA that allows for it to function as a cytosolic DNA sensor in innate immune responses, triggering activation of TBK1 and IRF-3, and subsequent production of type I interferons (6,7). Furthermore, ZBP1 can trigger the activation of NF-κB through interaction with the RIP homotypic interaction motif (RHIM) of RIPK1 and RIPK3 (8). ZBP1 binding to RIPK3 can also induce a process of programmed necrosis termed necroptosis (9). In contrast, its interaction with RIPK1 can repress necroptosis (10,11). The mRNA binding activity of ZBP1 is also thought to play a role in tumorigenesis. ZBP1 is repressed in metastatic breast cancer, which leads to dysregulation of mRNA targets involved in proliferation and metastasis (12,13). Alternate Names C20orf183; DAI; DLM-1; DLM1; DNA-dependent activator of IFN-regulatory factors; DNA-dependent activator of interferon regulatory factors; DNA-dependent activator of IRFs; Tumor stroma and activated macrophage protein DLM-1; Z-DNA binding protein 1; Z-DNA-binding protein 1; ZBP1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 44, 58 SOURCE: Rabbit