CST, 61024T, Fc gamma RIIIB/CD16B (F2D8G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FcGR3B. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Fc gamma RIIIB/CD16B (F2D8G) Rabbit Monoclonal Antibody (CST #61024) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Fc gamma RIIIB/CD16B (F2D8G) Rabbit Monoclonal Antibody recognizes endogenous levels of total FcγRIIIB/CD16B protein. This antibody does not cross-react with FcγRIIIA/CD16A. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val37 of human FcγRIIIB/CD16B protein. Background CD64 (FcgammaRI), CD32 (FcgammaRII), and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32, and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance, and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1). CD16B is expressed by neutrophils, while CD16A is expressed by NK cells, macrophages, and a subset of monocytes (4). CD16 is commonly used in combination with CD56 to characterize NK cells, with CD16 identifying NK cells capable of cytotoxicity (5). Alternate Names CD16; CD16A; CD16B; Fc fragment of IgG receptor IIIb; Fc fragment of IgG, low affinity IIIb, receptor (CD16b); Fc fragment of IgG, low affinity IIIb, receptor for (CD16); Fc gamma receptor III-B; Fc gamma receptor IIIb; Fc-gamma receptor IIIb (CD 16); Fc-gamma RIII; Fc-gamma RIII-beta; Fc-gamma RIIIb; FCG3; FCG3B; FCGR3; FCGR3A; FCGR3B; FcR-10; FcRIII; FcRIIIb; IGFR3; IgG Fc receptor III-1; Low affinity immunoglobulin gamma Fc region receptor III-B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50-100 Source/Isotype: Rabbit IgG