CST, 61178S, GPR52 (E9D8W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GPR52. Validated for Western Blotting,Immunofluorescence (Frozen). Available in 2 sizes. Highly specific and rigorously validated in-house, GPR52 (E9D8W) Rabbit Monoclonal Antibody (CST #61178) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 65216 . Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity GPR52 (E9D8W) Rabbit Monoclonal Antibody recognizes endogenous levels of total GPR52 protein. Nonspecific labeling of Mueller cells in fixed frozen mouse retina has been observed by immunofluorescence. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val249 of human GPR52 protein. Background G-protein coupled receptor 52 (GPR52), an orphan G-protein coupled receptor (GPCR), modulates dopaminergic and glutamatergic transmission in neuronal circuits responsible for cognitive function and emotion (1). GPR52 regulates those various brain functions through activation of cAMP-dependent pathways (2). Furthermore, GPR52 is a modulator of soluble mutant huntingtin (mHTT) levels in cells of Huntington's disease (HD) patients (3). Therefore, GPR52 is a novel therapeutic target for HD as well as psychiatric disorders (1-4). Initial studies have shown that a novel GPR52 antagonist reduces mutant HTT levels and rescues HD-associated phenotypes in cellular and mouse models (4). Alternate Names g protein-coupled receptor 52; G-protein coupled receptor 52; gpr52; mgc111751; otthump00000032861; probable G-protein coupled receptor 52 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 41 Source/Isotype: Rabbit IgG