CST, 61179S, NLRP10 (E5X5A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NALP10. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NLRP10 (E5X5A) Rabbit Monoclonal Antibody (CST #61179) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NLRP10 (E5X5A) Rabbit Monoclonal Antibody recognizes endogenous levels of total NLRP10 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile340 of human NLRP10 protein. Background The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family of proteins is a diverse family of cytoplasmic innate immune receptors. They are characterized by the presence of an amino-terminal effector domain, which is often either a caspase activation and recruitment domain (CARD) or a pyrin domain (PYD), followed by a NACHT domain and carboxy-terminal leucine-rich repeats (LRR) involved in recognition of pathogen-associated molecular patterns (PAMPs) (1). NLR proteins play a variety of roles during the innate immune response including pathogen sensing, transcriptional activation of proinflammatory cytokines through NF-κB, transcriptional activation of type I interferons through IRFs, and formation of inflammasomes leading to activation of inflammatory caspases (1-7). NLRP10 (NLR family pyrin domain-containing protein 10, PYNOD, NALP10) is the only NLR family member lacking the characteristic LRR repeats (8-12). In response to a variety of stimuli, including mitochondrial damage, the NLRP10 inflammasome is formed, leading to ASC speck formation and caspase-1-dependent cytokine release (12). Alternate Names CLR11.1; NACHT, leucine rich repeat and PYD containing 10; NACHT, LRR and PYD domains-containing protein 10; NAL10; NALP10; NLR family pyrin domain containing 10; NLR family, pyrin domain containing 10; NLRP10; NOD8; Nucleotide-binding oligomerization domain protein 8; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 10; PAN5; PYNOD Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG